Antibody sequence numbering and complementarity determining region (CDR) delimitation have wide applications in antibody engineering. They are generally obtained by mapping query sequences to the retrospective patterns. However, due to the enormous diversity of antibody sequences, novel patterns are often generated in antibody affinity maturation. They may not be recognized by the traditional methods. Antibody Region-Specific Alignment (AbRSA) integrates the biological insight of antibody region-specific feature with dynamic programming to improve the robustness of antibody numbering. Benchmarks show it has very good performance in numbering unusual antibodies and distinguishing between antibody and non-antibody sequences.
AbRSA web service is free for users, and, therefore, on an "as is" basis, without warranty of any kind.
AbRSA: A robust tool for antibody numbering (https://doi.org/10.1002/pro.3633). 2019, Protein Science